Xue Renfeng

Visiting Scholar

Xue Renfeng

 

Liaoning Academy of Agricultural Sciences  

Crop Research Institute  

Address: 84 DonglingRoad, Shenhe, Shenyang, 110161, China  

Tel: +86-24-31025226 (office)  

+86-158-4048-0642 (Cell phone)  

Email: xuerf82@hotmail.com  

 

 

 

Thesis Title of Ph. D.:    

Identification of Resistant Germplasm and Defence Mechanism Study in Common Bean( Phaseolus vulgaris L.) to Fusarium wilt  

                                                                                                           

Scholarship  

02/2014 –12/2014     Visiting scholar, TSU,TN, USA  

Advisor: Matthew Blair  

 

Education  

09/2009 – 07/2012     Ph.D. – Crop Germplasm Resources  

Chinese Academy of Agricultural Sciences, Beijing, P.R. China  

Advisor: Shumin Wang, Ph.D. ( wangshumin@caas.cn )   

Vice advisor: Zhendong Zhu, Ph.D. ( zhuzhendong@caas.cn )  

Technion: Lanfen Wang, (wanglanfen@caas.cn)         

09/2006 – 07/2009     Master – Biochemistry and Molecular biology  

Chinese Academy of Agricultural Sciences, Beijing, P.R. China  

                      Advisor: Fanrong Zeng, Ph.D. ( zengfanrong@caas.cn )  

09/2002 – 07/2006     Bachelor – Bioscience  

Shenyang Agricultural University, Liaoning, P.R. China  

 

Research Interests  

I am interested in the work for resistant resource screening and the mechanism between pathogen and host by scanning electron microscope and some molecular techniques such as enzyme assay, determination of biocontrol compound, Real-time PCR, cDNA-AFLP and RNAseq for transcriptional analysis, and 2D gels for proteomics analysis.  

 

Major Projects  

For master degree  

  1. Isolated a new protease inhibitor gene from the nematodes symbiotic bacteria Xenorhabdus bovienii and revealed the insecticidal bioactivity and mechanism against agricultural insect pests (Zeng F., Xue R. , et al., 2012, Pest Management Science; Xue R. F. et al., 2009, Biotechnology Bulletin (in Chinese); Jin D.J., Xue R. F. , et al., 2012, Chinese Journal of Biological Control (in Chinese) and 1 manuscript in preparation).    
  1. A new protease inhibitor gene designed as PIN1 was cloned from Xenorhabdus bovienii ;  
  1. The recombinant protein expressed in E. coli was purificated by affinity chromatography;  
  1. The insecticidal bioactivity and mechanism were determined against several important agricultural insect pests (aphids, green leaf bugs and whitefly) using the insect bioassay, measurement of enzyme activity and establishment of transgenic tobacoo.   
  1. Isolated a new toxin protein gene from Xenorhabdus bovienii and reveal the insecticidal bioactivity and mechanism (1 manuscript in preparation).  
  1. A new toxin protein gene designed as Xbtp-1 was cloned from Xenorhabdus bovienii ;  
  1. The recombinant protein expressed in E. coli was purificated by affinity chromatography;  
  1. The insecticidal bioactivity was determined against Helicoverpa armigera using the insect bioassay and measurement of digestive enzyme activity in midgut of insects;   
  1. Illumination of the insecticidal mechanism of Xbtp-1 showed that the Xbtp-1 recombinant protein enhanced the tissue degradation of the insect midgut by scanning electron microscopy.  

For Ph.D. degree   

  1. Isolated and identified Fusarium wilt pathogens and screened the resistant cultivar of common bean for Fusarium oxysporum f. sp. phaseoli (Fop).  
  1. 70 isolates of F. oxysporum f. sp. phaseoli were isolated and identified by                         phenotype, genotype (self-designed primer) and pathogenicity test, and an isolate of Fusarium oxysporum f. sp. phaseoli — Fop-DM01 exhibited strong virulence were used for screening of the resistant cultivars;  
  1. 362 cultivars of common bean were screened, and one cultivar (260205) showed stable resistance to Fop-DM01 isolate.  
  1. Designed a specific primer of F. oxysporum f. sp. phaseoli and distinguished the resistance levels of different bean cultivars by Real-time PCR. ( Xue R. F. , et al., 2012, Acta Agronomica Sinica)  
  1. A pair of specific primers was designed depended on a new transcriptional factor gene ( ftf1 genes) from Fop;  
  1. The capacity of pathogen colonization on different cultivars was distinguished by R eal time-PCR and verified by greenhouse experiment.  
  1. The study of the interaction between common bean and the pathogen Fop-DM01 isolate . (1 manuscript in preparation)  
  1. The organizational and celluar structure of infected roots and stems in susceptible cultivar BRB-130 which displayed by scanning electron micrographs and transmission electron micrographs degraded more seriously than that in resistant cultivar 260205.;       
  1. The activity of the plant defence-related emzymes, phenylalanine ammonia lyase (PAL) and peroxidase (POX), and the content of H 2 O 2 and O 2- as the signalling molecule of plant defence pathway in roots and stems of 260205 was higher than BRB-130;  
  1. Differentially expressed defence-related genes between the infected resistant 260205 and infected susceptibe BRB-130 were revealed by RNAseq and cDNA-AFLP analysis;  
  1. Proteomics analysis using 2D gels to discover the key proteins in the plant defence to the pathogen.  
  1. Cloning of two defence-responsed genes from the resistant cultivar 260205 named PvCaM1 encoding calmodulin and PvPOX1 encoding secretory peroxidase, separately. ( Xue R. F. , et al., 2012, Acta Agronomica Sinica (in Chinese) and 1 manuscript in preparation)  
  1. Cloning and analysis of the full-length cDNA and DNA of the two genes by Race and RT-PCR;  
  1. The expression level of the two genes in different organs and in response to biotic and abiotic stress by real time-PCR analysis (e.g. pathogen attack, wounding, cold, salt, drought and plant hormone);  
  1. Subcellular localization using GFP as a marker showed PvCaM1 was located in cytoplasm and PvPOX1 was secreted to extracellular matrix .  
  1. Resistance response to Fusarium oxysporum f. sp. phaseoli in common bean ( Phaseolus vulgaris L.) induced by salicylic acid (SA). ( Xue R. F. , et al., 2013, Journal of Plant Growth Regulation)  
  1. The resistance of susceptible cultivar BRB-130 was induced by SA treatment;  
  1. HPLC analysis indicated the content of free and conjugated SA in roots increased after SA treatment ;   
  1. PAL and POX activities in roots were both enhanced by SA treatment;  
  1. H 2 O 2 and O 2- content in roots increased after SA treatment;  
  1. The capacity of pathogen colonization in roots decreased after SA treatments detected by real time-PCR.  

 

Publications  

  1. F. Zeng, R. Xue , H. Zhang and T. Jiang. A new gene from Xenorhabdus bovienii and the protein of protease inhibitor encoded against Acyrthosiphon pisum . Pest Management Science. 2012,68:1345-1351.( equal contribution to the first author )  
  1. Xue R.F. , Wu J., Wang L.F., Matthew W. Blair, Wang X.M., Ge W.D., Zhu Z.D., Wang S.M. Salicylic acid enhances resistance to Fusarium oxysporum f. sp. phaseoli in common beans ( Phaseolus vulgaris L.). Journal of Plant Growth Regulation. 2014, 33:470-476.  
  1. Xue R.F. , Wu J., Chen M.L., Zhu Z.D., Wang L.F., Wang X.M., Matthew W. Blair, Wang S.M. Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean ( Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. phaseoli . Molecular Breeding. 2014, 34:855–870.  
  1. Xue R.F. , Wu J., Zhu Z.D., Wang L.F., Wang X.M., Wang S.M, Matthew W. Blair. Differentially expressed genes in resistant versus a susceptible common bean ( Phaseolus vulgaris L.) genotypes in response to Fusarium oxysporum f. sp. phaseoli . PLoS One, 2015 (accepted)  
  1. Xue R.F. , Wang X.J., Li M., Zeng F.R. Research Progress of Toxic Protein from Entomopathogenic Nematodes Symbiotic Bacteria. Biotechnology Bulletin, 2009(4):21 ～ 24. (in Chinese)    
  1. Xue R.F. , Zhu Z.D., Huang Y., Wang X.M., Wang L.F., Wang S.M. Quantification of Fusarium oxysporum f. sp. phaseoli Detected by Real-Time Quantitative PCR in Different Common Beans Cultivars, Acta Agronomica Sinica, 2012, 38(5): 791 ～ 799.  
  1. Xue R.F. , Zhu Z.D., Wang X.M, Wang L.F., Wu X.F., Wang S.M. Cloning and Expression Analysis of Fusarium Wilt Resistance-related Gene PvCaM1 in Common Bean ( Phaseolus vulgaris L.), Acta Agronomica Sinica, 2012, 38(4): 606 ～ 613. (in Chinese)  
  1. Xue R.F. , Wu J., Zhu Z.D., Wang L.F., Wang X.M., Ge W.D., Wang S.M. Salicylic acid increases resistance to Fusarium oxysporum f. sp. phaseoli in common beans ( Phaseolus vulgaris L.). Journal of Plant Genetic Resources, 2014,15 (5): 1138 ～ 1143. (in Chinese)  
  1. Li L.R., Xue R.F. , Wang X.J., Zeng F.R. Predation of Nesidiocoris tenuis to Trialeurodes vaporariorum and Plutella xylostella , Journal of Agricultural University of Hebei, 2008,31(1):83 ～ 87. (in Chinese)  
  1. Wang X.J., Xue R.F. , Li M., Zeng F.R. Progress on α-Amylase Inhibitors from Microorganism, Biotechnology Bulletin, 2009(7):52 ～ 55. (in Chinese)  
  1. Jin D.J., Xue R.F. , Zhang H.Q., Zeng F.R., Dong S.L. Insecticidal Activity of a Protease Inhibitor from Xenorhabdus bovienii strain BJFS526 to Pea Aphids ( Acyrthosiphon pisum Harris), Chinese Journal of Biological Control, 2012, 28(4):508 ～ 513. (in Chinese)  
  1. Zhuang Y., Xue R.F. , Ge W.D. Insecticidal Activity Study of the Crude Extract from Xenorhabdus spp. strain Sy1a, Liaoning Agriculture Sciences, 2014 (4):15 ～ 18.(in Chinese)  
  1. Xue R.F. , Zhao Y., Zhuang Y., Chen J., Wang Y.J., Li T., Jin X.M., Li L.R., Ge W.D. Effect of Several Herbicides on Weeds and Phenotype of Mung Bean ( Vigna radiata ) in Field, Henan Agriculture Sciences, 2015, in press, (in Chinese).  
  1. Xue R.F. , Chen J., Zhao Y., Wang Y.J., Li T., Zhuang Y., Jin X.M., Li L.R., Ge W.D. Effect of different planting densities and seed coating agents on the growth and physiological characteristics of adzuki beans ( Vigna angularis ). Journal of Agricultural University of Hebei, 2015, in press, (in Chinese).  
  1. 葛维德  

 

Meetings/Abstracts  

  1. Li M., Zeng F.R., Xue R.F. , Wang X.J. Cloning and sequence analysis of P450 gene fragment from Streptomyces ahygroscopicus . Annual Conference of Chinese Plant Protection Society, Guilin, Guangxi, November 23-25, 2007. (in Chinese)  
  1. Xue R.F. , Wang X.J., Zeng F.R., Wu Q.J. Preliminary study of protease inhibitors from Xenorhabdus bovienii to control whitefly. Annual Conference of Chinese Plant Protection Society, Chongqing, October 28-31, 2008. (in Chinese)  
  1. Wang X.J., Xue R.F. , Zeng F.R. Growth inhibition of α -amylase inhibitor against Helicoverpa armigera . Annual Conference of Chinese Plant Protection Society, Chongqing, October 28-31, 2008. (in Chinese)  
  1. Xue R.F. , Zeng F.R., Li M. Cloning and expression of a protease inhibitor gene from Xenorhabdus bovienii and the bioactivity against aphids. Seminar of Chinese Youth in Plant Protection, Nanchang, Jiangxi, May 16-18, 2008. (in Chinese)  
  1. Xue R.F. , Zhu Z.D., Wang X.M., Wu X.F., Wang S.M. Screening of resistant resources to Fusarium wilt in common bean ( Phaseolus vulgaris L.). Annual Conference of Chinese Plant Protection Society, Xiamen, Fujian, July 3-7, 2010. (in Chinese)  
  1. Xue R.F. , Zhu Z.D., Wang X.M., Wu X.F., Wang S.M. Differentially expression of the resistance related gene in common bean against Fusarium wilt pathogen by c DNA-AFLP analysis. Annual Conference of Chinese Plant Protection Society, Yichang, Hubei, August 18-24, 2011. (in Chinese)  
  1. Chen M. L., Wang L.F., Xue R.F. , Xu X.X., Wang S.M. Development of mapped simple sequence repeat markers from common bean ( Phaseolus vulgaris  L.) based on genome sequences. Chinese Academy of Crop Science Society, Chendu, Sichuan, 2011.(in Chinese)  
  1. Xue R.F. , Ge W.D., Wang S.M. Salicylic acid Regulates Resistance to Fusarium oxysporum f. sp. phaseoli in Common Beans ( Phaseolus vulgaris L.). Yangling,Shanxi, 2013. (in Chinese)  

 

 

Manuscript in Preparation  

  1. Xue R.F. , Wu J., Zhu Z.D., Wang L. F., Matthew W. Blair, Wang S.M. Cloning and characterization of a novel secretory, root-expressed peroxidase gene from common bean ( Phaseolus vulgaris L.) infected by Fusarium oxysporum f. sp. phaseoli.  
  1. Xue R.F. , Wu J., Zhu Z.D. and Wang S.M. Different transcript profiling of defence–related genes induced on challenge with the root-infecting fungal pathogen Fusarium oxysporum f. sp. phaseoli in common bean.  
  1. Xue R.F. , Zeng F.R., Cui J.J., Ma Y., Li L.R., Wang X.J. Cloning and characterization of a novel gene from the bacteria of Xenorhabdus bovienii strain BJFS526 against Helicoverpa armigera .   
  1. Zeng F.R., Liu Z, Xue R.F. , Zhang Y. Characterization of the bacteria of Xenorhabdus bovienii strain BJFS526 and a 45 kDa protein.   
  1. Xue R.F. , Jin D.J., Zhang H.Q., Li L.R., Wang X.J., Zeng F.R. The study of bioactivity of the recombinant protein of the protease inhibitor gene Xbpi-1 from Xenorhabdus bovienii BJFS526. (in Chinese)  

 

Patents  

  1. Zeng F.R., Xue R.F. Expression and application of a new protease inhibitor gene from bacteria. Code: ZL200810094371.5. (in Chinese)  
  1. Zeng F.R., Xue R.F. A new method for determination of the bioactivity of the biopesticide. Code: 2008100943700.0. (in Chinese)  
  1. Wang S.M., Xue R.F. , Zhu Z.D., Wang L.F., Wang X.M. A primer pair for identification of Fusarium oxysporum f. sp. phaseoli and its application. Code: ZL201210033056.8. (in Chinese)  
  1.  

 

Internship Experience and Social Activities  

2006-2009   Committee member of Class committee. Responsible for class activities and daily routine.  

              Counselor for the students of high school. Responsible for helping them to solve the problem they have in their study.   

2008        Beijing Olympic city volunteers. Responsible for directing the foreign visitors and athletes to travel in Beijing, and supply any required help for them in the time of 2008 Beijing Olympic games.  

 

Honors and Awards  

  1. Learning Scholar Award; Technology Innovation Award; Student Scholarship; Excellent Student  
  1. Outstanding Communist Youth League Member; Student Scholarship; Excellent Learning Award  
  1. Outstanding Communist Youth League Leader; Outstanding Student Leader  

2008  Excellence Award of Mid-term Evaluation for Master Candidate  

2011  Excellence Award of Mid-term Evaluation for Doctor Candidate  

 

English skills  

 

Reading  

Listening  

Writing  

Speaking  

Good  

Acceptable  

Good  

Acceptable